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Conjugated bilirubin can be measured in the same reaction by omitting the solubilizing agent (since it is already in a conformation where the diazo dye can react – this is because conjugation itself disrupts those hydrogen bonds)
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[DOI] [PubMed] [Google Scholar] López-Pedrosa J.M., Manzano M., Baxter J.H., Rueda R
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