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In situ tyrosinase activity was visualized by addition of 60μl of 50mM potassium phosphate buffer (pH 6.8) containing 2mM DOPA (3, 4 dihydroxy phenylalanine) and 4mM MBTH reagent (3-Methyl-2-Benzothiazolinone hydrazone hydrochloride) for 1 hour at 37℃
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Prdx6 overexpression also abolished HG-induced ferroptosis, as reflected in the inhibition of iron accumulation and increased expression of SLC7A11 and GPX4
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