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2.3 Western blot and antibodies The equal amounts (30 μg) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)

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Succinyl-CoA plays an important role in the production of energy from lipids and proteins and is also required for the synthesis of hemoglobin, the oxygen-carrying pigment in red blood cells (3)