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In a recent in vitro and in vivo study, using Lactobacillus and Bifidobacterium conducted by Amaretti et al., the authors identified that given the colon colonization by administered probiotics, it is conceivable that their overall protective effect could be related to activities taking place at the intestinal level, i.e

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Finally, antibody binding was detected using 80 μL/well diluent SuperSignal Elisa Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific)