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For seabream ( Sparus aurata ), fish (92.37± 6.06 g) were acclimated for 9 days in sea water (with salinity of about 40‰) open flow through tanks (270L), under natural ambient temperature in the range of 22-24˚C and divided for 3 treatments as follows: fed fish that were fed twice a day (Raanan food, Israel) with food containing 46% protein ad libitum , or fish that were food deprived for 48 h (SD) or 7 days (LD) prior to sampling