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Preclinical studies have indicated that combining the MCT1 inhibitor AZD3965 with anti-PD-1 therapy lowers lactate release into the TME and boosts anti-tumor immunity (118)

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For quantification, an aliquot of dissolved sample was analyzed using an Applied Biosystems MDS SCIEX 4000 QTRAP liquid chromatography-tandem mass spectrometry system (AB Sciex, Foster, CA, USA)