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Melatonin exhibits a significant impact on intestinal damage by elevating the expression of intestinal tight junction proteins (ZO-1, Occludin, and Claudin-1), reducing intestinal permeability, and modulating gut microbiota composition by decreasing the abundance of Firmicutes and increasing the abundance of Bacteroidetes phyla (88)

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Noteworthy, in both cases, a characteristic band at 345 nm could be distinguished at the beginning of the reaction (Figure 4)