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Both cells were cultured at 37°C with 5% CO 2 , generally subcultured every 2–3 days, and regularly tested for mycoplasma using mycoblue® mycoplasma Detector (D101-02, Vazyme)
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In addition, the protective effects of both a recombinant adiponectin injection in ob/ob mice and metformin administration against NAFLD have been attributed, at least in part, to the inhibition of hepatic TNFα expression 24,25
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