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primers for CCNE1 : 5′-GCAGCAGAATTCGCCACCATGCCGAGGGAGCGCAGGG-3′ and 5′-GCAGCAGGATCCTCACGCCATTTCCGGCCCGC-3′] and inserting the resulting PCR product into pLVX-TetOne-Puro (Clontech Laboratories, 631849) between EcoRI and BamHI sites
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Size:100 Grams
The colonic muscle is the most oxygenated region with 7%–10% oxygen levels (Schwerdtfeger et al., 2019)
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