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Transfection was carried out using Lipofectamine 3000 Kit on cells at 50% confluence for 6 hours, followed by protein extraction 48 hours post-transfection

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Cycle-dependent modulation of mitochondrial dynamics—including biogenesis, fusion–fission balance, and oxidative phosphorylation efficiency—likely contributes to phase-specific differences in substrate utilization observed during rest and exercise, and in health and disease as well [53,54,55]