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In the final step of this pathway, CTNNB1 binds to the transcription factors TCF/LEF-1 ( Figure 1 Beyond its classical pro-tumourigenic functions, Wnt/β-catenin activation has been found to be associated with T cell-depletion across tumour entities including ACC ( Mechanistically, Wnt signalling regulates peripheral T cell activation and differentiation of CD4 + T helper and CD8 + cytotoxic T cells (CTLs) ( + T cells ( regs ) ( Conversely, the production of IL-1β by tumour-associated macrophages has been shown to result in the phosphorylation and the subsequent stabilisation of β-catenin in colon cancer ( Despite the prominent role of this pathway in ACC, a systematic study of Wnt/β-catenin signalling and its potential interconnections with tumour-infiltrating immune cells has not been conducted so far
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