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Surface Preparation
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The membranes were blocked for 120 min with 5% (w/v) milk dissolved in 0.1% Tween-20 in TBS at room temperature, and then were incubated overnight at 4°C with the following primary antibodies: Caspase12 (1:1000, Abcam, United States), IRE1α (1:1000, Abcam, United States), GRP78 (1:1000, Abcam, United States), XBP-1 (1:1000, Abcam, United States), CHOP (1:1000, Cell Signaling Technologies, United States), Ace-tubulin (1:2000, Cell Signaling Technologies, United States), Tau (1:1000, Abcam, United States), MAP2 (1:2000, Cell Signaling Technologies, United States), myelin basic protein (MBP) (1:1000,Cell Signaling Technologies, United States), and GAPDH (1:10000, Bio-world, United States)
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