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Downregulation of microRNA-196a inhibits stem cell self-renewal ability and stemness in non-small-cell lung cancer through upregulating GPX3 expression
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Cold RNA refolding buffer (10 mM Tris-HCl at pH 7, 100 mM KCl, 10 mM MgCl 2 ) was then added, and samples were incubated at room temperature (20–25 °C) for 20–30 min
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