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The relative mRNA expression was calculated from the quantification cycle values (Cq) of targeted genes by normalizing with the Cq values of the reference gene, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), using the comparative threshold method (2 −ΔCT ) [29]

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Furthermore, the removal of the endothelium and treatment with potassium channel blockers reduced the vasorelaxation response, indicating the critical role of potassium channels (Santos et al., 2022)