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The Mass Spectrometry data have been deposited in the PRIDE Proteomics database under accession code PXD043133

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The significant increase in homoarginine suggested that the decreased arginine levels in GLUT1OE cells were likely due to its conversion to homoarginine, and not due to decreased biosynthesis from citrulline, since two upstream rate limiting enzymes argininosuccinate synthase 1 (ASS1) and argininosuccinate lyase (ASL) were increased by GLUT1OE in CD8 + GLUT1OE CARs and CD19.28ζ-GLUT1 respectively, nor from decreased arginine demand, as transcription of the main arginine transporter SLC7A1 was significantly upregulated (Fig