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Proteins were then separated via 8% SDS-PAGE (Servicebio, Wuhan, China), transferred to PVDF membranes, and blots were then blocked for 2 h at room temperature with 5% non-fat milk prior to incubation overnight with appropriate primary antibodies at 4°C

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Our previous study also identified a significant association between the NNMT genetic variant at rs1941404 and T2D in the Chinese Han population (Li et al., 2018)