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Does the patient display any abnormal postures such as Schiff-Sherrington posture (extended rigid forelimbs, flaccid paralysis of the hind limbs) that may signify severe spinal shock or a severed spinal cord

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Estimated Duration:10 Minutes

Lysates were sonicated 10 times with 1-s pulses at 80% amplitude on a Fisher Scientific Model 120 Sonic Dismembrator (Thermo Fisher Scientific), then centrifuged at 16,000 g at room temperature (for 9 M urea) or at 4°C (for RIPA lysis) for 15–20 min