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Reconstitution Solution:3ML

One hour after plating, the plating media was removed and replaced with maintenance media consisting of Neurobasal-A (Gibco), 0.5x B27 supplement (Gibco), 0.5x Glutamax (Gibco), 35 mM D-glucose (Sigma), 100 U/ml penicillin-G and 100 µg/ml streptomycin