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Once stabilized, HIF-α translocates to the nucleus, dimerizes with HIF-1β/Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT), and forms a HIF transcription factor to promote cellular pathways influencing proliferation and malignant progression (156–158, 164)
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The blots were then incubated with an ECL reagent and the expression levels of the target proteins were detected using a chemiluminescence imaging analysis system (Clinx Science Instruments Co., Ltd.)
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