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However, they present notable limitations, including high maintenance costs, long generation times (∼3 months to sexual maturity), reduced feasibility for large-scale screening, constraints in high-resolution live imaging (often requiring sacrifice or invasive methods), and increasingly stringent ethical considerations ( in vitro systems offer controllability, short experimental cycles, and are amenable to high-throughput screening (Kobar et al., 2021), they lack the tissue microenvironment, cell–cell interactions, systemic feedback, and metabolic capacity necessary for accurately predicting in vivo efficacy and toxicity (Koechling et al., 2010
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Control parasite survival was over 95% in both aerobic and anaerobic growth conditions
