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Niacinamide (vitamin B3, technically not peptide but frequently combined in peptide formulations ) addresses pigmented dark circles through multiple mechanisms including inhibiting melanosome transfer from melanocytes to keratinocytes reducing visible pigmentation, anti-inflammatory effects decreasing post-inflammatory hyperpigmentation from rubbing or allergies, and barrier strengthening improving overall skin health and resilience

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To quantify the quercetin-glutathione conjugates released by HAEC, a standard curve was constructed with purified standards (Fig