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NAD+ reduction increases methylation of certain gene promoters, which makes DNA methylation-sensitive factors detach and causes chromatin to compact and silence genes

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2.5 Competitive anti-CD38 staining NK92 cells were first stained using the Fixable Aqua Dead Cell Kit (Thermofisher), followed by incubation with either with titrated amounts of Darzalex (Daratumumab) (Johnson & Johnson), or plasma from patients or healthy control and incubated for 10 mins at room temperature