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Millipore-Sigma R9379 Enzyme-based creatinine assay kit ( see Note 1) 10 kDa spin columns ( see Note 2) Ultrapure (18 MΩ) water Sterile PBS: 140 mM NaCl, 10 mM sodium phosphate, pH 7.4 1” 22 Ga stainless-steel gavage needle with 1.25 mm ball tip