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Extracellular vesicles are associated with C–reactive protein in sepsis

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In vitro PLA2G2F activity assay Sample preparation and in vitro reaction Control, PLA2G2F isoform 2- and PLA2G2F-H110Q-overexpressing RT112 cells were harvested from 85% confluent 15-cm plates in lysing buffer (50 mM Tris-HCl (pH 8.0), 150 mM sodium chloride (NaCl), 0.5% Triton X-100 and 5% glycerol (v/v)) containing protease inhibitor (Thermo Scientific, A32955)